Bioanalytical Chemistry

Timetable

15 hours Lecture, 30 hours laboratory.

Time and dates of lectures and laboratories will be announced later.

Module aim (aims)

• Providing knowledge on the determination of bioanalytes  used in biomedical diagnostics.
• Providing knowledge of the theoretical basis of methods used in the determination of bioanalytes.
• Providing knowledge about the operating principles of individual analytical instruments and understanding their limitations and effectiveness in specific types of analysis.
• Developing the ability to properly interpret measurement results and evaluate the analytical method.
• Developing skills in writing reports and using literature sources.
• Developing communication skills and individual and team work.

Pre-requisites in terms of knowledge, skills and social competences (where relevant)

Organic chemistry (basic level)

Syllabus

Lectures:

Week 1: The subject and tasks of bioanalytical chemistry

Week 2: Analytical process, procedures and measurement techniques.

Week 3: Overview of bioanalytes of diagnostic importance.

Week 4: Basic information about proteins and their application in bioanalysis (immunochemistry).

Week 5: Basic information about nucleic acids and their application in bioanalysis.

Week 6: Biopolymer separation techniques (centrifugation, GE, HPLC).

Week 7: Modern methods of bioanalyte detection.

Laboratories:

Each student will perform the analyses independently. During the course, the following analytes will be determined: glucose, cholesterol, urea, DNA, microelements.

Reading list

1. Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer "Biochemitsry" 2012

2. Victor A. Gault, Neville H. McClenaghan "Understanding Bioanalytical Chemistry" 2009

3. D.A.Skoog et al., Principles of Instrumental Analysis, Thomson Brooks/Cole 2007