Introduction to Fluorescence

Timetable

15 h of lectures combined with calculations, practicals/demonstrations (in the lab) and problem-based learning

Module aim (aims)

Transfer of knowledge in the field of physical chemistry, photophysics, photochemistry and emission spectroscopy;

Familiarization with basic terms and equipment (includig laser-based devices) used in fluorescence spectroscopy with broad scope of applications in: chemistry, physics, biology and medicine

 

Pre-requisites in terms of knowledge, skills and social competences (where relevant)

Syllabus

1. Basic terms related to fluorescence: spectral range, emission spectrum, fluorescence and phosphorescence quantum yield, excited states, lifetimes, kinetics, etc...

2. Light sources (incl. the laser), equipment for fluorescence spectroscopy - basic principles and practical uses

3. Equipment for fluorescence measurements and its components (spectroscopy and microscopy)

4. Time-resolved fluorescence and its challenges 

5. Singlet oxygen 

6. Practicals - applications of fluorescence methods in industry, lasers, single molecule fluorescence, fluorescence microscopy

Reading list

Obligatory:

Joseph R. Lakowicz "Principles of Fluorescence Spectroscopy", 3rd edition, Springer New York 2006