General information

Course type AMUPIE
Language English
Module lecturer prof. UAM dr hab. Anetta Zioła-Frankowska
Lecturer's email
Lecturer position Professor
Faculty Faculty of Chemistry
Semester 2024/2025 (summer)
Duration 30
USOS code 02-BSMPA


Duration: lecture - 15 h, lab - 15 h , SUM=30 h

Timetable-lecture 15h+ lab 15h=30h*4 groups=120 h

Number of maximum students per class for one group-  4 students; number of groups- 4

Module aim (aims)


To learn about spectrophotometric methods used to analyse the chemical and physical properties of real sample with various environmental matrix.

To acquire laboratory skills required for performing a range of chemical and physicochemical analyses by using spectrophotometric methods.

To understand the methods used to assess the accuracy and precision of the analytical techniques performed in lab.

To design of chemical experiments with using spectrophotometric methods and equipment.


Describe and use principal analytical methods , especially spectrophotometric for chemical analysis.

Interpret and report data derived from chemical experiments/analysis in a meaningful way.

Demonstrate competency in the use of standard techniques of chemical analysis and the treatment of experimental data.

Apply the spectrophotometric instrumental methods to chemical analysis of real samples.

Pre-requisites in terms of knowledge, skills and social competences (where relevant)

Basic knowledge in the field of analytical chemistry, calculations in chemistry, rules in working with basic analytical equipment.


Basic spectrophotometric methods in practise course is designed to acquaint students with the theory and application of spectrophotometric methods for analysis standards and real samples with various matrix. The lectures will cover the basic theory of Spectroscopy; light (a particle, a wave or both), wave properties, how radiation and matter interacts,  electromagnetic spectrum, what do spectroscopists measure, UV-VIS spectrophotometry, absorbance, chromophores, Beer’s Law, energy level diagrams for molecules, instruments for measuring absorption.  The aim of the laboratory is to give students experience in performing spectrophotometric analysis of the chemical composition characteristics in real samples, like water or biological samples. The objective of the laboratory exercise is to become familiar with a typical spectrophotometric analysis and to examine the effect of an interfering substance.   This course builds on the laboratory experience, which focuses on hands-on experience with modern analytical instrumentation. The laboratory exercise should successfully facilitated an understanding of spectrophotometric analysis techniques and the effect of an interfering substance. The theory will be presented in lecture and the students will gain experience in a discussion and lab. During lecture and lab it will be discuss the basic principles that are necessary to understand measurement made with electromagnetic radiation, particularly those dealing with the absorption of UV, visible,  radiation.

Reading list

  1. Chemistry / John McMurry, Robert C. Fay. McMurry, John., Fay, Robert C. New Jersey : Printice Hall, 2004.
  2. Analytical chemistry : a modern approach to analytical science / ed. by J.-M. Mermet, M. Otto, M. Valcárcel ; founding eds R. Kellner, H. M. Widmer. Kellner, Robert A. Red., Mermet, Jean-Michel. Red., Widmer, H. Michael. Red., Valcárcel Cases, Miquel. Red., Otto, Matthias. Red.Weinheim : Wiley-VCH, cop. 2004.
  3. Analytical instrumentation : performance, characteristics and quality / Graham Currell. Currell, Graham., John Wiley & Sons. New Delhi : Wiley India, 2010.
  4. Fundamentals of analytical chemistry / Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch. Skoog, Douglas A.., Holler, F. James. Autor, Crouch, Stanley R. West, Donald M. Autor, Cengage Learning. Beoston : Cengage Learning, © 2022. 
  5. Fundamentals of chromatography / Harold Gomes Cassidy. Cassidy, Harold Gomes. New York ; London : Interscience Publishers, 1961.
  1. Fundamentals of general, organic, and biological chemistry / John McMurry, Mary E. Castellion. McMurry, John , Castellion, Mary E., Prentice Hall (Upper Saddle River). Upper Saddle River : Prentice Hall, cop. 2006.