Basic molecular methods

Timetable

Module aim (aims)

1. To deep knowledge of nucleic acids and proteins and their relationships; transfer of knowledge concerning basic molecular methods in biology and medicine.
2. Acquisition of skills by students to independent choice of basic molecular methods and its application and data interpretation.
3. Developing teamwork skills.

Pre-requisites in terms of knowledge, skills and social competences (where relevant)

Basic knowledge of proteins and nucleic acids. Critical thinking and teamwork skills.

Syllabus

SYLLABUS – A COURSE DESCRIPTION
I. General informaion
1. Course name: Basic molecular methods
2. Course code: 01-EPA-MOLMETHO-PIE
3. Course type (compulsory or optional):optional
4. Study programme name: AMU-PIE
5. Cycle of studies (1st or 2nd cycle of studies or full master’s programme): 2nd cycle of studies
6. Educational profile (general academic profile or practical profile): general academic
7. Year of studies (if relevant): I
8. Type of classes and number of contact hours (e.g. lectures: 15 hours; practical classes: 30 hours):
lectures: 10 hours
Laboratoria: 20 hours
9. Number of ECTS credits: 3
10. Name, surname, academic degree/title of the course lecturer/other teaching staff:
dr hab. Małgorzata Wojtkowska Prof.UAM, woytek@amu.edu.pl
Amit Nagwani, aminag@amu.edu.pl
11. Language of classes: english
12. Online learning – yes (partly – online / fully – online) / no: Lectures and practical course partially online on microsoft teams platform.
II. Detailed information
1. Course aim (aims)
1. To deep knowladge of nucleic acids and proteins and their relationships; transfer of knowladge concerning basic molecular methods for these molecules for the biology and medicine.
2. Acquisition of skills by students to independent choice of basic molecular methods and its application and data interpretation.
3. Developing teamwork skills.
2. Pre-requisites in terms of knowledge, skills and social competences (if relevant)
1. Basic knowladge of proteins and nucleic acids. 2. Critical thinking and teamwork skills.
3. Course learning outcomes (EU) in terms of knowledge, skills and social competences and their reference to study programme learning outcomes (EK)
Course learning outcome symbol (EU) On successful completion of this course, a student will be able to: Reference to study programme learning outcomes (EK)
Efekt_01 To depict the basic knowladge concerning basic information of the structure and function of the nucleic acids and proteins. K_W01, K_U12
Efekt_02 To explain the meaning of the analysis of nucleic acids and proteins in environmental studies. K_W01, K_W02, K_W04, K_U07
Efekt_03 To characterise the basic molecular methods dedicated to the nucleic acids and proteins.. K_W16, K_W14, K_W01
Efekt_04 To arrange the experiment using analysis of the nucleic acids and proteins in the biology and medicine. K_W01, K_W05, K_W18, K_U04
Efekt_05 cooperating in group to perform experiment based on the basic molecular methods; to perform data analysis and write the conclusions. K_W05, K_W14, K_W21, K_U01, K_K01, K_K02
Efekt_06 criticlly evaluate the experimental data presented by the other students. K_U05, K_K04
4. Learning content with reference to course learning outcomes (EU)
Course learning outcome symbol (TK) Course learning content Course learning outcome symbol (EU)
TK_01 The basic knowledge of the nucleic acids structure, function and the meaning in the biology and medicine. Efekt_01, Efekt_02, Efekt_02
TK_02 The basic knowledge of the nucleic acids structure, function and the meaning in the biology and medicine. Efekt_01, Efekt_02, Efekt_03
TK_03 The use of knowledge of nucleic acids and methods for their analysis as: cloning, PCR and its applications, Real-time PCR; microarrays, genotyping (SNP i RLFP); Sanger and NGS sequencing in the biology and medicine Efekt_03, Efekt_04, Efekt_05, Efekt_05
TK_04 The use of knowledge of proteins and methods for their analysis as: protein isolation and purification, elctrophoresis immunodewtection, Elisa test, microscope technics, mass spectrometry mas, protein expression in model organisms, in the biology and medicine Efekt_03, Efekt_04, Efekt_05, Efekt_06
5. Reading list
Books
1.Berg JM., Tymoczko JL., Stryer L: Biochemistry, 2018
2. Bruce Alberts,Dennis Bray,Karen Hopkin,Alexander D Johnson,Julian Lewis,Martin Raff,Keith Roberts,Peter Walter. Essential cell biology, Garland Science, 2013.

III. Additional information
1. Teaching and learning methods and activities to enable students to achieve the intended course learning outcomes (please indicate the appropriate methods and activities with a tick or/and suggest different methods)
Teaching and learning methods and activities
Lecture with a multimedia presentation yes
Interactive lecture yes
Problem – based lecture
Discussions yes
Text-based work
Case study work yes
Problem-based learning
Educational simulation/game
Task – solving learning (eg. calculation, artistic, practical tasks)
Experiential work yes
Laboratory work yes
Scientific inquiry method yes
Workshop method
Project work
Demonstration and observation yes
Sound and/or video demonstration yes
Creative methods (eg. brainstorming, SWOT analysis, decision tree method, snowball technique, concept maps)
Group work yes
2. Assessment methods to test if learning outcomes have been achieved (please indicate with a tick the appropriate methods for each LO or/and suggest different methods)
Assessment methods Course learning outcome symbol
EK_1 EK_2 EK_3 EK_4 EK_5 EK_6
Written exam yes
Oral exam
Open book exam
Written test yes yes yes yes
Oral test
Multiple choice test
Project
Essay
Report
Individual presentation
Practical exam (performance observation)
Portfolio
3. Student workload and ECTS credits
Activity types Mean number of hours spent on each activity type
Preparation for classes 15
Reading for classes 5
Essay / report / presentation / demonstration preparation, etc. 5
Project preparation
Term paper preparation 15
Exam preparation 10
Total hours 50
Total ECTS credits for the course 3
4. Assessment criteria according to AMU in Poznan grade system
Very good (bdb; 5,0):
Good plus (+db; 4,5):
Good (db; 4,0):
Satisfactory plus (+dst; 3,5):
Satisfactory (dst; 3,0):
Unsatisfactory (ndst; 2,0):

Reading list

1. Berg JM., Tymoczko JL., Stryer L: Biochemistry, 2018
2. Bruce Alberts,Dennis Bray,Karen Hopkin,Alexander D Johnson,Julian Lewis,Martin Raff,Keith Roberts,Peter Walter. Essential cell biology, Garland Science, 2013.