Blood Physiolgy

Timetable

Lectures (15 h = 7x2 +1x1)

March 03 - April 28, on Fridays, from 12.15 to 13.45, lecture hall "L" (first floor)

 

Laboratory (15 h = 3x 5h)

Lab1  - May 31, 14.00-17.45 (BZ2)

Lab 2 - June 05, 8.00-11.45 (F3)

Lab3  - June 14, 16.00-19.45 (F3).

Module aim (aims)

1. Provide knowledge about blood rheology, hemodynamics, and modulation of blood flow in circulation system in health and disease.
   2. Presenting dynamics of pre- and postnatal hematopoiesis and characteristics of multipotent hematopoietic cells and hematopoietic niches.
   3. Provide knowledge about hematopoietic disorder types, symptoms, and causes, including anaemia, leukaemia, multiple myeloma, and lymphoma.
   4. Describe of objectives, types, trends, and outcomes of hematopoietic stem cells transplantation in blood and bone marrow diseases.
   5. Presenting human red blood cells as a cell model for screening of biological activity of natural and synthetic compounds, including nanomaterials.
   6. Provide knowledge about human erythrocytes as carriers for a wide range of drugs and for diagnostic purposes.
   7. Providing knowledge about preservation and storage of blood and blood components, transfusion risks, and artificial blood industry.
   8. Improving skills in laboratory work with human blood cellular components - erythrocytes
   9. Developing skills in planning experiments with whole blood and isolated blood cellular components, and in vitro hemocompatibility assays with data analysis.

Pre-requisites in terms of knowledge, skills and social competences (where relevant)

cell biology, animal physiology and anatomy

Syllabus

1. Overview of blood and blood cellular components properties and modifications of blood rheology by external and internal factors in health and disease
2. Prenatal and postnatal hematopoiesis development and functions - the properties that define multipotent hematopoietic stem cells and mesenchymal stem cells in hematopoetic niches.
3. Bone marrow structure and functions in health and diseases - aplastic anemia, acute and chronic leukemia, lymphoma, radiation and chemical injury, aging-relating.
4. Hematopoietic stem cells transplantation in blood and bone marrow diseases.
5. Human red blood cells as a model for screening of activity of blood-contacting compounds, including nanomaterials
6. Application and safety of red blood cells as carriers for drugs and for diagnostic purposes.
7. Assays for in vitro hemocompatibility evaluation of materials for biomedical applications.

Reading list

Books

1. Salama M.: Atlas of Diagnostic Hematology, Elsevier, Amsterdam, 2020
   2. Hoffman R.: Hematology - basic principles and practice, Elsevier, London, 2017

 Papers  

1. Moradi S., Jahanian-Najafabadi A., Roudkenar M.H. (2019): Blood Rheology: Key Parameters, Impact on Blood Flow, Role in Sickle Cell Disease and Effects of Exercise, Front Physiol, 10: 1329
   2. Julien E., Omar R., Tavian M. (2016): Julien E., Omar R., Tavian M. Origin of the hematopoietic system in the human embryo, FEBS Letters, 590: 3987–400
   3. Morrison S.J., Scadden D.T. (2914): The bone marrow niche for haematopoietic stem cells, Nature, 505: 329
   4. Margolis L., Sadovsky Y. (2019): The biology of extracellular vesicles: the known and unknowns , PloseOne, 17(7): e3000363
   5. Qadri S.M., Bissinger R., Solh Z., Oldenborgf P.A. (2017): Eryptosis in health and disease: A paradigm shift towards understanding the (patho)physiological implications of programmed cell death of erythrocytes, Blood Review, 31: 349-361
   6. Staffas A., Burgos da Silva M., van den Brin M. (2017): The intestinal microbiota in allogeneic hematopoietic cell transplant and graft-versus-host disease, Blood, 129 (8): 927-933
   7. Xu J., Wang Y., Guttorp P., Abkowitz J.L. (2018): Visualizing hematopoiesis as a stochastic process., Blood Advances, 2: 2637-2645
   8. Xing F., Hu F., Yang J., Pan L., Xu J.and Jingjun X (2019): Structural and functional studies of erythrocyte membrane-skeleton by single-cell and single-molecule techniques, J Innovat Optic Health Sciences, 12: 1830004
   9. Koleva L., Bovt E., Ataullakhanov F. ,3 and Elena Sinauridze (2020): Erythrocytes as Carriers: From Drug Delivery to Biosensors, Pharmaceutics, 12: 276
   10. Moradi S., Jahanian-Najafabadi A., Roudkenar M.H. (2019): Artificial Blood Substitutes: First Steps on the Long Route to Clinical Utility, CliniCal MediCine insights: Blood disorders, 10: 1329